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KMID : 0604520110370030265
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Journal of the Society of Cosmetic Scientists of Korea
2011 Volume.37 No. 3 p.265 ~ p.273
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Antioxidative and Inhibitory Activities on Tyrosinase of Hippophae rhamnoides Leaf Extracts
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Kim Jung-Eun
Chae Kyo-Young
Park Soo-Nam
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Abstract
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In this study, the antioxidative and inhibitory effects on tyrosinase and elastase of Hippophae rhamnoides (H. rhamnoides) leaf extracts were investigated. The ethyl acetate fraction of H. rhamnoides extracts showed more effective free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50 = 4.68 μg/mL). Reactive oxygen species (ROS) scavenging activity (OSC50) of the aglycone fraction in the luminol-dependent Fe3+-EDTA/H2O2 system was 0.19 μg/mL. The aglycone fraction exhibited more prominent cellular protective effects (τ50, 133.3 min at 10 μg/mL) in the 1O2-induced photohemolysis of human erythrocytes. The inhibitory effect (IC50) of the aglycone fraction on tyrosinase was 54.86 μg/mL, and more effective than arbutin known as whitening agent. These results indicate that fractions of Hippophae rhamnoides extract can be used as antioxidants in biological system, particulaly skin exposed to UV radiation by quenching and/or scavenging 1O2 and other ROS, and protecting cellular membranes against ROS.
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KEYWORD
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Hippophae rhamnoides, antioxidant, tyrosinase, elastase, cellular protective effect
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